Human Papillomavirus (28 Jenis) Kit Detéksi Genotyping (Fluorescence PCR)
Ngaran Produk
HWTS-CC004A-Human Papillomavirus (28 Jinis) Kit Detéksi Genotyping (Fluorescence PCR)
Épidemiologi
Kit ngagunakeun sababaraha metode deteksi fluoresensi asam nukléat amplifikasi (PCR).Primer sareng panyilidikan anu khusus pisan dirarancang dumasar kana urutan target gen L1 HPV.Panyilidikan husus dilabélan ku FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC / HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 44). , 45, 54, 56, 68, 82) jeung ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) dina 5', sarta grup quencher 3' nyaeta BHQ1 atanapi BHQ2.Salila amplifikasi PCR, primers sareng panyilidikan khusus ngabeungkeut kana sekuen target masing-masing.Nalika énzim Taq papanggih panyilidikan kabeungkeut kana runtuyan udagan, éta exerts fungsi 5' tungtung exonuclease pikeun misahkeun fluorophore reporter ti fluorophore quencher, ku kituna sistem ngawaskeun fluoresensi bisa nampa sinyal fluoresensi, nyaeta, unggal waktu DNA. strand digedékeun, molekul fluoresensi kabentuk, anu ngawujudkeun sinkronisasi lengkep akumulasi sinyal fluoresensi sareng formasi produk PCR, ku kituna pikeun ngahontal deteksi kualitatif sareng genotyping asam nukléat tina 28 jinis papillomavirus manusa dina conto sél exfoliated cervical. .
Saluran
| FAM | 16,58,53,73,6,26,40 |
| VIC/HEX | 18,33,51,59,11,81,43 |
| ROX | 31,66,52,39,83,61,42 |
| CY5 | 56,35,45,68,54,44,82 |
Parameter Téknis
| Panyimpenan | ≤-18 ℃ Dina poék |
| Kahirupan rak | 12 bulan |
| Jenis spésimén | Sél exfoliated cervical |
| Ct | ≤25 |
| CV | ≤5,0% |
| LoD | 25 salinan / réaksi |
| Instrumén lumaku | Gampang Amp Real-time Fluoresensi Isothermal Deteksi System (HWTS1600)
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MA-6000 Real-Time kuantitatif termal Cycler
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Aliran Gawé
Pilihan 1.
réagen ékstraksi dianjurkeun: Macro & Micro-Test Sampel Reagen Release (HWTS-3005-8).
Pilihan 2.
réagen ékstraksi dianjurkeun: Makro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) jeung Macro & Micro-Test Otomatis Nukléat Asam Extractor (HWTS-3006).
